Abstract: According to the features of disease and environment in flue-cured tobacco floating seedling stage of Zunyi region，spatial electric field preventing disease system which built by biological effect electrostatic field generator was set in floating seedling shed.Root disease，air borne disease，tobacco virus disease and climate spot disease were investigated.Results showed that occurring rate of root disease was significantly lower than control group in spatial electric field environment，occurring rate of air borne disease was also lower than control group，and spatial electric field didn′t lead to tobacco climate spot disease.Investigation results also showed that occurrence degree of tobacco virus disease in experimental group was lower than that in control group，but it could not be clearly attributed to the role of space electric field，it should be the result of comprehensive prevention and control.Investigation results proved that spatial electric field preventing disease technology could be used for the prevention of root diseases and air borne diseases in flue-cured tobacco floating seedling shed.Comprehensive physical protection measures should be adopted to prevent tobacco virus disease.