Abstract: Effective botanical-insecticides were screened from endophytic fungi extracts.Insecticidal activity that 70 fermentation extracts of 44 kinds endophytic fungi which were separated from 9 kinds of plants to cinnabar leaf mites and peach aphid were determined by glass immersion method and drop method respectively.Results showed that the various extracts（2 mgmL）to hyalopterus amygdali had higher mortality rates，24 h contact toxicity to cinnabar leaf mite was not obvious under 0.5 mgmL，48 h fatality rates were improved in different degree.There were 6 fermentation extracts of endophytic fungi had high insecticidal activity including CQ-5，CQ-8，DLD-2，GT-10，GWT-1 and JK-2.High activity strain MQZ-4-L was screened out from extracts of the test.It had stronger insecticidal activity to cinnabar tetranychus mites whose LD50 of 48 h calculated value was 0.482 9 mgmL.This study provided a basis for the discovery of insecticidal activity of new lead compounds.