Abstract: Practical teaching is an effective way to consolidate theoretical knowledge and deepen theory understanding，and an important link to cultivate high quality talents with innovative consciousness.The key to cultivate applied talents that could adapt to development of modern agriculture for agronomy specialty is to change concept and to strengthen construction of school enterprise cooperation practice base.Combined with an example of practice teaching of school enterprise cooperation in Hunan Agricultural University，background of talent training base construction of school enterprise cooperation，preliminary results of construction and future construction measures were analyzed.