Abstract: Taking whole bulb，heart bud，shoot tip and seed of Bulus tulipae as explant，complete and virus-free plants were obtained by tissue culture technology.Test results showed that success rate of sterilization of explants was 92%，subculture multiplication factor was up to 2.6，rooting rate was 91.3% and transplanting survival rate of tissue culture seedlings was 95%.This research results provided a strong technical support for sustainable development of Bulus tulipae industry.