光慈菇组培种苗商品化生产技术应用试验

Application Test on Commercial Production Technology of Bulus tulipae Tissue Culture Seedlings

摘要: 以光慈菇的全鳞茎、心芽、茎尖和种子为外植体，利用植物组培快繁技术培养出完整的无毒植株。试验结果表明，外植体灭菌处理成功率为92%，继代增殖系数最高达2.6，生根率为91.3%，组培苗移栽成活率为95%。该研究成果为光慈菇产业的可持续发展提供了有力的技术支撑。

Abstract: Taking whole bulb，heart bud，shoot tip and seed of Bulus tulipae as explant，complete and virus-free plants were obtained by tissue culture technology.Test results showed that success rate of sterilization of explants was 92%，subculture multiplication factor was up to 2.6，rooting rate was 91.3% and transplanting survival rate of tissue culture seedlings was 95%.This research results provided a strong technical support for sustainable development of Bulus tulipae industry.