Abstract: In order to study effect of hormone treatment on seed germination and discuss effective hormone concentration ratio of promoting seed germination of Taxus chinensis var.mairei，taking Taxus chinensis var.mairei seeds as experiment material，using orthogonal test design of L25（53），choosing three hormones GA3，6-BA and 2，4-D，concentration gradient of 0，0.25，0.5，0.75 and 1.0 mgL，seed germination rate，germination index，vigor index and mean germination time were measured.Test results showed that germination rate of A1B2C2 was the highest，which was 85.33%.Germination index（6.95）and vigor index（19.25）of A1B1C1 were the highest.Average germination time of A4B2C5 was the shortest，which was 6.04 d.Comprehensive analysis，GA3 0.75 mgL+6-BA 0.25 mgL had the best effect on improving germination rate and shortening germination time.In addition to average germination time，extreme values of other indexes were GA3＞6-BA＞2，4-D，extreme values of mean germination time were 6-BA＞GA3＞2，4-D.GA3 and 6-BA had extremely significant effect（P＜0.01）and significant effect（P＜0.05）on seed germination rate，main effect was GA3＞6-BA＞2，4-D.GA3 and 6-BA had extremely significant effect（P＜0.01）on seed germination index，vigor index and mean germination time，main effect was GA3＞6-BA＞2，4-D.Main effect of mean germination time was 6-BA＞GA3＞2，4-D.Therefore，GA3 was the key factor affecting seed germination rate，germination index and vigor index of Taxus chinensis var.mairei.6-BA was the key factor to promote seed germination of Taxus chinensis var.mairei.Effect of 2，4-D was not obvious.