Abstract: In order to effectively suppress effect of external vibration on measurement results by three-dimensional static stiffness instrument and enhance inherent response frequency of three-dimensional static stiffness instrument，design method of tree-dimensional static stiffness instrument which was based on Hypermorph shape optimization was put forward.Taking maximization of first order natural frequency as target optimization function，shape optimization model of three-dimensional static stiffness instrument was created.Static stiffness of lathe was tested by optimized three-dimensional static stiffness tester through LabVIEW virtual technology.Measurements of natural frequency of vibration of the first 6 order and LabVIEW software system showed that the first order natural frequency of optimized three-dimensional static stiffness tester was obviously higher than that before optimization.It had better vibration suppression performance.And optimized three-dimensional static stiffness meter was more accurate.The method was of practical significance for design of engineering structures with external disturbances.And the use of LabVIEW virtual technology could improve automation and efficiency of experimental test.