Abstract: Experimental Design and Statistical Analysis is a required course for graduate students of agricultural machinery specialty.Learning of the course lays foundation for the follow-up research of graduate students.However，there are some problems in the course of teaching，such as disconnection of theory and practice，little interaction between teachers and students，and complexity of calculation.Teaching content of the course was discussed on the basis of practical needs.Key points of the software were introduced combined with requirements of teaching program and functions and characteristics of Matlab data processing system.Some ideas and measures for teaching reform based on combination of teachers′ scientific research subjects and practical problems of enterprises were put forward.Practice proved that students′ interest in learning and ability to solve problems have been improved.