Abstract: Pests and diseases are common phenomenon in greenhouse vegetable cultivation.Prevention of pests and diseases could effectively increase the productivity of greenhouse vegetable crops.Biotechnology could effectively reduced the use of traditional pesticides and pollution of pesticides on land in greenhouses，which ensured vegetable healthy.Application of biotechnology were analyzed in prevention and control of pests and diseases in greenhouse vegetables.Biotechnology should be actively used to prevent and control vegetable pests and diseases.