笋用竹组培快繁及其种苗产业化

Tissue Culture and Rapid Propagation of Shoot-producing Bamboo and Seeding Industriulization

摘要: 以苏麻竹等笋用竹的顶芽、侧芽和根等为外植体，通过诱导培养形成脱毒不定芽，通过组培快繁技术，培养出完整的无毒植株。试验结果表明：外植体灭菌处理成功率达78%，诱导不定芽的最适培养基为MS+6-BA3.0+IBA0.2+NAA0.1，不定芽继代增殖的最适培养基为MS+6-BA2.0+IBA0.2+NAA0.1，增殖系数为2.32，生根率达91%，移栽成活率达93%，为笋用竹种苗提供了一种新型快速的繁殖方式。

Abstract: Terminal buds，lateral buds and roots of some kinds of shoot-producing bamboo like Dendrocalamus latiflorus Munro were taken as explants，adventitious buds were successfully induced by tissue culture，after rapid propagating，complete and virus-free plants were obtained.Results showed that explants sterilization success rate was as high as 78%.Optimal medium for adventitious buds induction was MS+6-BA3.0+IBA0.2+NAA0.1，optimizing medium for subculture multiplication was MS+6-BA2.0+IBA0.2+NAA0.1，and subculture multiplication factor was up to 2.32，rooting rate was 91% and transplanting survival rate was 93%，is could provide an effect way for rapid propagation of virus-free shoot-producing bamboo seedlings.