Abstract: Single factor tests were conducted with process conditions of electrolysis time（5，10，15，20，25，30 and 35 min），pH（2，4，6，8，10 and 12）and current density（2，4，6，8，10 and 12 mA/cm2）by using of 13.275 mg/L methylene blue solution to simulate agricultural organic wastewater，orthogonal test of 3 factors and 3 levels was designed with electrolysis time，pH and current density as factors，COD removal rate and decolorization rate in wastewater were taken as reference standard for treatment effect.Optimal parameter combination for treating organic wastewater in electrolysis test using Fe2O3-GAC as three-dimensional electrode was obtained.Results showed that the best process parameters for three-dimensional electrolytic treatment of wastewater were electrolysis time of 30 min，current density of 8 mA/cm2 and pH value of 6.Under optimal parameters，COD removal rate reached to 59.7%，and decolorization rate reached to 88.5%，that a better treatment effect was achieved.