Abstract: Pepino melon has characteristics of strong adaptability，high nutritional value，high yield and high benefit.It has been promoted throughout country since its introduction，and it plays an important role in strategic adjustment of modern agricultural planting structure.The use of plant stem tip detoxification technology and tissue culture factory seedling breeding technology is the key technology carrier to promote development of large-scale，high-quality，high-efficiency，low-cost，and annual production of pepino melon seedlings.Detoxification and rapid propagation technology of pepino melon were systematically sort out.Research progress of 3 detoxification technologies and 4 virus detection technologies for three major virus diseases of pepino melon，TMV，PMV and CMV，were introduced.Seedlings of pepino melon in China were analyzed.Specific problems and shortcomings in research of virus-free and rapid propagation technology were analyzed.Compared with research progress in related fields abroad，some suggestions were put forward for future research direction of virus-free and rapid propagation of pepino melon seedlings in China，with a view to help Chinese agricultural，scientific Biotech workers and agricultural technology companies provide references.