莲雾组培快繁种苗及其主要栽培技术

Tissue Culture and Rapid Propagation Technology of Wax Apple and its Cultivation Techniques

摘要: 以莲雾的茎尖、腋芽等为外植体，通过诱导培养形成脱毒不定芽，通过组培快繁技术，培养出完整的无毒植株。试验结果表明：外植体灭菌处理成功率达93%，诱导不定芽的最适培养基为MS+6-BA 2.0+IBA 0.2+GA3 0.2，增殖系数为3.2；不定芽出根的最适培养基为MS+IBA 2.0+VB1 0.2，生根率达92.8%；移栽成活率达96%，为莲雾种苗提供了一种新型快速的繁殖方式。

Abstract: Using some kinds of wax apple，with terminal buds，lateral buds and other parts as explants，adventitious buds were successfully induced by tissue culture.After rapid propagating，complete and virus-free plants were obtained.Results showed that explants sterilization success rate was as high as 93%.Optimal medium for adventitious buds induction was MS+ 6-BA 2.0+ IBA 0.2+ GA3 0.2，and the subculture multiplication factor was up to 3.2.Optimizing rooting medium was MS+ IBA 2.0+VB1 0.2.The rooting rate was 92.8% and transplanting survival rate was 96%.The study provided an effect way for rapid propagation of virus-free wax apple.