Abstract: Carbonate rock areas are susceptible to karst development due to special topography，geomorphology，hydrological conditions，etc.In the development and utilization of water resources in such areas，it is necessary to first identify distribution and development of curtain karst，and carry out existing seepage paths detecting and tracking，thus ensuring smooth implementation and safe and stable operation of water resources development and utilization engineering construction.A water conservancy in the southern part of Yunnan-Guizhou Plateau was taken as research object.The geophysical and geophysical comprehensive detection and analysis of karst seepage path was carried out by electric method and electromagnetic wave CT method combined with drilling.Detection results showed that for complex karst water flow systems，comprehensive detection method could effectively detect karst seepage channel，and detection results could provide reliable basis for treatment of seepage.