Abstract: In order to explore the best concentration combination of water and fertilizer supply for three-dimensional strawberry cultivation in Guanzhong，Shaanxi Province，soilless culture medium（peat∶vermiculite∶inorganic salt=1∶2∶1）was used as culture system，W1F1、W1F2、W1F3、W2F1、W2F2、W2F3 and CK（clear water，100% water content of substrate）were treated with two water contents（W1，W2）and three water-soluble fertilizer concentrations（F1，F2，F3），effects of different gradient treatment on plant growth，dry matter accumulation and water utilization rate were studied in the three-dimensional cultivation of strawberry.Results showed that W1F2（30% water content in the substrate and 0.1% water-soluble fertilizer concentration）did not cause excessive plant growth，accumulated more dry matter in the underground part，and root shoot ratio was higher than that of high water and fertilizer irrigation mode.