Abstract: Mushroom chaff contains high nutrients and it is a fertilizer with high application value.Taking Yuzhi No.16 white sesame as experimental object，mushroom chaff was applied to soil to compare effects of different treatment methods on soil nutrients，white sesame yield，contents of iron and zinc in roots and grains.Results showed that application of mushroom chaff could increase content of soil total nitrogen，total potassium and organic matter，had little effect on content of soil total phosphorus，but could significantly increase content of available phosphorus in soil.Yield of white sesame treated with mushroom chaff was good，1000 grain mass and yield per plant were better than those of the control.Contents of trace elements in roots and grains of white sesame treated with mushroom chaff were higher，contents of iron and zinc were better than the control.According to comprehensive indexes，effect was the best when application amount of mushroom chaff was 60 t/hm2.