Abstract: Effects of different red light（R）and blue light（B）ratios on growth and physiology of blueberry tissue culture seedlings were studied.Ten different red and blue light ratios（i.e.1∶1，1∶2，1∶3，1∶4，1∶5，1∶6，1∶7，1∶8，1∶9 and 1∶10）were used to treat blueberry tissue culture seedlings，and content of photosynthetic pigment were determined.Results showed that contents of chlorophyll a and total chlorophyll in T4 treatment were the largest；content of chlorophyll b in T10 treatment was the highest；T3 treatment had the highest carotene content.Different proportion of blue light and red light could change growth and physiological indexes of blueberry tissue culture seedlings.