Extraction Conditions Optimization and Antioxidant Capacity Research of Polyphenols from Inonotus obliquus
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摘要:
以桦褐孔菌菌核为试验材料，利用超声−微波协同萃取法提取了桦褐孔菌样品中的多酚，采用单因素和多因素试验优化了桦褐孔菌多酚的提取条件，测定了多酚的抗氧化能力。试验结果显示，超声−微波协同萃取法提取桦褐孔菌多酚的优化条件是乙醇体积分数50%、料液比1∶60、微波功率400 W、超声波功率350 W、提取时间240 s，此条件下的多酚提取率为2.943%。该方法提取的多酚具有良好的抗氧化能力，对DPPH自由基清除率的
IC 50值为9.03 μg/mL，对羟自由基清除率的IC 50值为2.03 mg/mL。该方法是一种实用、有效、快速的提取桦褐孔菌多酚的方法。-
关键词:
- 桦褐孔菌 /
- 多酚 /
- 超声−微波协同萃取法 /
- 抗氧化能力
Abstract:Polyphenols were extracted by ultrasonic-microwave co-extraction with sclerotia of
Inonotus obliquus ．Extraction conditions of polyphenols fromInonotus obliquus were optimized by single factor and multi-factor experiments, and antioxidant capacity of polyphenols was determined．Results showed that optimal extraction conditions were as follows: ethanol volume fraction of 50%, solid-liquid ratio of 1∶60, microwave power of 400 W, ultrasonic power of 350 W and extraction time of 240 s．Under these conditions, extraction rate of polyphenols was 2.943%．Polyphenols extracted by this method had good antioxidant capacity．TheIC 50 of DPPH free radical scavenging was 9.03 μg/mL, and theIC 50 of hydroxyl free radical scavenging was 2.03 mg/mL．This method is a practical, effective and rapid method for the extraction of polyphenols fromInonotus obliquus ． -
图 1 乙醇体积分数对桦褐孔菌多酚提取率的影响
Figure 1. Effects of ethanol volume fraction on extraction rate of polyphenols from Inonotus obliquus
图 2 料液比对桦褐孔菌多酚提取率的影响
Figure 2. Effects of solid-liquid ratio on extraction rate ofpolyphenols from Inonotus obliquus
图 3 微波功率对桦褐孔菌多酚提取率的影响
Figure 3. Effect of microwave power on extraction rate of polyphenols from Inonotus obliquus
图 4 超声波功率对桦褐孔菌多酚提取率的影响
Figure 4. Effect of ultrasonic power on extraction rate of polyphenols from Inonotus obliquus
图 5 提取时间对桦褐孔菌多酚提取率的影响
Figure 5. Effect of extraction time on extraction rate of polyphenols from Inonotus obliquus
图 6 桦褐孔菌多酚对DPPH自由基的清除能力
Figure 6. Scavenging activity of polyphenols from Inonotus obliquus on DPPH free radical
图 7 桦褐孔菌多酚对羟自由基的清除能力
Figure 7. Scavenging ability of polyphenols from Inonotus obliquus on hydroxyl radical
表 1 因素水平
Table 1. Factor and level
水平 因素 乙醇体
积分数A/%料液比B/
（g∶mL）微波
功率C/W超声波
功率D/W提取
时间E/S1 40 1∶50 250 210 120 2 50 1∶60 300 350 180 3 60 1∶70 350 560 240 4 70 1∶80 400 700 300 
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表 2 正交试验结果
Table 2. Orthogonal test results
序号 因素 提取率/% A B C D E 1 1 1 1 1 1 1.255 2 1 2 2 2 2 2.486 3 1 3 3 3 3 2.086 4 1 4 4 4 4 1.889 5 2 1 2 3 4 2.037 6 2 2 1 4 3 2.156 7 2 3 4 1 2 1.834 8 2 4 3 2 1 2.243 9 3 1 3 4 2 2.213 10 3 2 4 3 1 2.282 11 3 3 1 2 4 1.808 12 3 4 2 1 3 1.756 13 4 1 4 2 3 2.292 14 4 2 3 1 4 1.355 15 4 3 2 4 1 1.779 16 4 4 1 3 2 1.745 K1 1.929 1.949 1.741 1.550 1.890 K2 2.067 2.070 2.014 2.207 2.070 K3 2.015 1.877 1.974 2.037 2.072 K4 1.793 1.908 2.074 2.009 1.772 R 0.274 0.193 0.333 0.657 0.300
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