Abstract: The aim of this study was to explore appropriate density and time for waterless live transportation of Urechis unicinctus．Physiological stress and quality indicators of samples in control group, low-density group, medium-density group and high-density group were analyzed at different transport time．Results showed that concentration of cortisol in three density groups was significantly higher than that in control group（P<0.05）．Water holding capacity and lactic acid contents changed significantly after treatment of 24 h（P<0.05）．Transportation density stress had a greater effect on glucose content．Glucose content in high-density group was significantly higher than other two density groups at 12 to 60 h（P<0.05）．There was no significant difference in SOD activities between different densities at 36 h and 60 h, and change of malondialdehyde showed the same trend（P>0.05）．Recommended transport density should not exceed 1.0 kg/L during waterless live transportation to better maintain quality of Urechis unicinctus．