Abstract: Perfect practice teaching is objective requirement for cultivating application-oriented talents in colleges and universities, and school-enterprise collaborative teaching is an important way to cultivate high-level applied talents．Significance, current situation, and shortcomings of school enterprise cooperation practical teaching mode in bioengineering major in higher education institutions was analyzed．From perspective of mutual development among students, schools, and enterprises, teaching reform ideas were proposed to promote rational development of applied talent cultivation in bioengineering major．