Abstract: In order to verify effects of multi-year mixed application of biogas slurry and mushroom residue on yield and quality of tomato and soil environment in solar greenhouse, a single variable method was used to take "Provence" tomato as test material．The yield, quality and soil physiochemical properties of tomato under four treatments of conventional fertilization（CK）, biogas slurry returning to field（BS）, oyster mushroom residue returning to field（MR）and combined application of biogas slurry and mushroom residue（BM）were studied．Compared with CK, BS, MR and BM treatments, yield and soluble sugar content of tomato were increased by 19.33%, 14.32% and 31.49%, respectively．Contents of soil organic matter, available nitrogen, available phosphorus and available potassium were increased, and soil environment of tomato planting was improved．Diversity of fungi community was increased, and diversity of archaea and fungi community was decreased．BS or BM treatment could not only increase tomato yield, but also to some extent improve basic physical and chemical properties of soil, change microbial community structure in tomato rhizosphere soil, and promote crop nutrient absorption and utilization．