Abstract: At present, domestic bagging edible mushroom liquid strain inoculation method is mainly manual and semi-automatic machine inoculation．The production efficiency is low and mechanical failure rate is high, which has not yet reached requirements of bagging edible mushroom factory production．In response to problems of difficult inoculation positioning, low inoculation efficiency and high equipment failure rate of bagging edible mushroom liquid strain inoculation, a kind of bagging edible mushroom liquid strain inoculation equipment was developed, that could realize automatic transportation, positioning, clamping, vial pressing, capping, spray inoculation, capping, testing and conveying．It could complete fully automatic continuous liquid strain inoculation of fungus seeds．This equipment could effectively reduce manual work intensity and avoid contamination caused by outside environment and caused by long time of opening and closing the lid．It could improve efficiency of liquid strain inoculation and quality of mushroom production, and realize automatic inoculation of liquid strain in bags of edible mushroom．The minimum inoculation capacity of machine was 3236 sticks/hour, and the average was 3256 sticks/hour >3000 sticks/hour．The maximum coefficient of variation of inoculum consistency was 1.9%, and the average was 1.78%＜2%, which met requirements of mission statement．No failure occurred in 56 hours of test work, meeting Mean Time Between Failure（MTBF）> 50 hours．The test drilling depth was 56.3～57.8 mm, and average value was 57.33 mm．There was no failure or problem in strength and stiffness of this machine during whole experiment．It showed that strength and stiffness of mechanical part of machine was designed to meet production requirements．Mechanical performance was stable and reliable．