Abstract: Effects of mushroom residue cow dung manure with different bran meal as auxiliary material on growth, yield, quality and soil physical and chemical properties of tobacco were studied．Field experiment was conducted to study effects of peanut bran（OFH）, cottonseed bran（OFM）, rapeseed bran（OFY）and soybean bran（OFD）mushroom residue cow dung manure（3.0 t/hm²）on soil organic matter, available nutrients, tobacco plant growth, chemical composition and quality on the basis of 90% local conventional chemical fertilizer application．Results showed that, fertilizer effect of different bran meal was different when 3.0 t/hm² was applied to soil．Nitrogen content and single fresh leaf weight of OFH treatment were the highest, and phosphorus and potassium content of tobacco leaf was similar to that of other bran meal．OFH treatment had the highest proportion of premium tobacco and output value of tobacco leaves, which had no significant difference with OFD treatment, but were significantly higher than OFY and OFM treatment．Different treatments had no adverse effects on chemical constituents of flue-cured tobacco, and OFH treatment had better coordination of chemical constituents．There was no significant difference in comprehensive evaluation score of flue-cured tobacco sensory quality among the four treatments, and OFH was the highest, followed by OFY．Effect of improving nutrition and growth of tobacco plant and improving yield and quality of flue-cured tobacco was better to make mushroom residue cow dung manure with fungus residue from peanut bran as auxiliary material．