Abstract: To solve the problems of low efficiency，bottle error and uneven of liquid spawn inoculation，a high-speed precise liquid spawn inoculation devices was designed.Structure and principle of this inoculation device were clarified.Jet flux and combined nozzle parameters were analyzed emphatically.And the performance tests were carried on.Taking inoculation accuracy，bottle error and repetitive error as indicators，injection pressure，nozzle diameter and jet time were studied.Test and analysis results indicated that injection pressure，nozzle diameter and jet time had highly significant effect on inoculation effect，and the optimum parameters were 0.17 MPa for injection pressure，0.4 mm for nozzle diameter and 0.52 s for injection time.Test proved that inoculation accuracy was more than 99%，bottle error was less than 3%，and repeat error was less than 0.4 g.The indicators meet the demand of edible fungi factory production.