Abstract: In order to explore disinfection technology of alkaline electrolyzed water for living tilapia，effects of pH value，salinity of alkaline electrolyzed water and treating time of tilapia in alkaline electrolyzed water on bacteria reduction rate of tilapia epidermis by using fresh tilapia were evaluated.On basis of single factor experiment，response surface method was used to optimize sterilization process，and optimal process conditions were obtained： 10 of pH value of alkaline electrolyzed water，8‰ of salinity，and 60 min of treating time of living tilapia in alkaline electrolyzed water.Under these conditions，bacteria reduction rate of tilapia epidermis was up to 94.1%，indicated that alkaline electrolyzed water has a stronger bactericidal effect on living tilapia.